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Literature summary for 7.1.1.2 extracted from

  • Nakamaru-Ogiso, E.; Matsuno-Yagi, A.; Yoshikawa, S.; Yagi, T.; Ohnishi, T.
    Iron-sulfur cluster N5 is coordinated by an HXXXCXXCXXXXXC motif in the NuoG subunit of Escherichia coli NADH:quinone oxidoreductase (complex I) (2008), J. Biol. Chem., 283, 25979-25987.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
H101A the NADH dehydrogenase subcomplex (NuoEFG subcomplex) in the cluster N5 mutant is unstable and dissociates from complex I. Recovery of these mutant NuoCDEFG subcomplexes by expressing the His-tagged NuoCD subunit, which has a high affinity to NuoG. At temperatures around -270°C no cluster N5 signals are found in the cluster N5 mutant Escherichia coli
H101A/C114A the NADH dehydrogenase subcomplex (NuoEFG subcomplex) in the cluster N5 mutant is unstable and dissociates from complex I. Recovery of these mutant NuoCDEFG subcomplexes by expressing the His-tagged NuoCD subunit, which has a high affinity to NuoG. At temperatures around -270°C no cluster N5 signals are found in the cluster N5 mutant Escherichia coli
H101C the NADH dehydrogenase subcomplex (NuoEFG subcomplex) in the cluster N5 mutant is unstable and dissociates from complex I. Recovery of these mutant NuoCDEFG subcomplexes by expressing the His-tagged NuoCD subunit, which has a high affinity to NuoG. At temperatures around -270°C no cluster N5 signals are found in the cluster N5 mutant Escherichia coli
additional information cluster N5 knock-out (DELTAN5) mutant: the NADH dehydrogenase subcomplex (NuoEFG subcomplex) in the cluster N5 mutant is unstable and dissociates from complex I. The data confirm that, cluster N5 has a unique coordination with His(Cys)3 ligands in subunit NuoG. Recovery of these mutant NuoCDEFG subcomplexes by expressing the His-tagged NuoCD subunit, which has a high affinity to NuoG. At temperatures around -270°C no cluster N5 signals are found in the cluster N5 mutant Escherichia coli
W221A mutant is used as a control subcomplex carrying wild-type clusters. At temperatures around -270°C a cluster N5 signals is detected in the control Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe iron-sulfur cluster N5 is coordinated by an HXXXCXXCXXXXXC motif in the NuoG subunit Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of the NuoCDEFG subcomplex Escherichia coli

Synonyms

Synonyms Comment Organism
complex I
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 Escherichia coli
NADH:quinone oxidoreductase
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 Escherichia coli